CNRS Université Bordeaux

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Uranium neurotoxicology

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The main objective of this study was to describe the mechanisms of toxicity of natural uranium, after a continuous exposure of 7 days, on human SH-SY5Y cells presenting a dopaminergic phenotype. Cell viability was assessed for the first time on this cell type, showing that the cytotoxicity of uranium only occurred at high concentrations (> 125 μM), far above the expected values for uranium in the blood, even after occupational or accidental exposure. The SH-SY5Y differentiated cells were continuously exposed to 1, 10, 125 or 250 μM of natural uranium for 7 days and the quantitative subcellular distribution of uranium was studied using micro-PIXE imaging. Subcellular imaging revealed that uranium was located in well-defined perinuclear regions of the cytoplasm, suggesting its accumulation in organelles. Uranium was not detected in the nucleus of differentiated cells. In addition, the expression of genes linked to the dopaminergic pathway has been quantified. The expression of the monoamine oxidase B gene (MAO-B) is statistically significantly reduced after exposure to uranium while the other genes were not modified. This original finding suggests that the inhibition of dopamine catabolism, but also other MAO-B substrates, may be selective neurotoxicity effects of uranium (Carmona et al., 2018). In addition, another component of this project involved the isotopic analysis of uranium in neuronal cells. In collaboration with the CEA teams, we revealed the isotopic fractionation of uranium after cellular uptake suggesting the existence of a high-affinity transport protein for uranium (Paredes et al., 2016 and 2018).

Figure. Optical and micro-PIXE imaging of single SH-SY5Y cells after 7-day continuous exposure to 125 µM uranium. (A) Bright field optical microscopy. (B) Epifluorescence optical microscopy of Hoechst 33542 nuclear dye. (C) Nucleus position derived from the epifluorescence image (false color, for overlay). (D) Phosphorus distribution. (E) Uranium distribution. (F) Overlay image of nucleus and uranium distribution. (Carmona et al., 2018).


Laboratory of Protein-Metal Interactions (LIPM), Institute of Biosciences and Biotechnologies of Aix Marseille (BIAM), UMR7265 CEA – CNRS - Aix Marseille Univ, CEA Cadarache.

CEA, DRF, Biosciences and Biotechnologies Institute (BIAM), Bagnols-sur-Cèze.

Den – Service d’Etudes Analytiques et de Réactivité des Surfaces (SEARS), CEA, Université Paris-Saclay, Gif sur Yvette.


Cytoplasmic aggregation of uranium in human dopaminergic cells after continuous exposure to soluble uranyl at non-cytotoxic concentrations
Carmona A., Porcaro F., Somogyi A., Roudeau S., Domart F., Medjoubi K., Aubert M., Isnard H., Nonell A., Rincel A., Paredes E., Vidaud C., Malard V., Bresson C., Ortega R. (2021), NeuroToxicology, 82, 35-44. [link]

Isotopic variations of copper at the protein fraction level in neuronal human cells exposed in vitro to uranium
Paredes Paredes E., Malard V., Vidaud C., Avazeri E., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2019), The Analyst , in press. [link]

Deciphering the uranium-target proteins in human dopaminergic SH-SY5Y cells
Vidaud C., Robert M., Paredes E., Ortega R., Avazeri E., Jing L., Guigonis J.M., Bresson C., Malard V. (2019), Archives of Toxicology, in press. [link]

Uranium exposure of human dopaminergic cells results in low cytotoxicity, accumulation within sub-cytoplasmic regions, and down regulation of MAO-B
Carmona A., Malard V., Avazeri E., Roudeau S., Porcaro F., Paredes E., Vidaud C., Bresson C., Ortega R. (2018), NeuroToxicology, 68, 177-188. [pubmed] [link]

Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
Paredes E., Avazeri E., Malard V., Vidaud C., Reiller P.E., Ortega R., Nonell A., Isnard H., Bresson C. (2018), Scientific Reports, 8, 17163. [link]

A new procedure for high precision isotope ratio determinations of U, Cu and Zn at nanogram levels in cultured human cells : what are the limiting factors?
Paredes E., Avazeri E., Malard V., Vidaud C., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2018), Talanta, 178, 894-904.[link]

Evidence of isotopic fractionation of natural uranium in cultured human cells

Paredes E., Avazeri E., Malard V., Vidaud C., Reiller P., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2016), Proc. Natl. Acad. Sci. USA., 113, 14007-14012. [pubmed] [link]