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2021
Cytoplasmic aggregation of uranium in human dopaminergic cells after continuous exposure to soluble uranyl at non-cytotoxic concentrations
Carmona A., Porcaro F., Somogyi A., Roudeau S., Domart F., Medjoubi K., Aubert M., Isnard H., Nonell A., Rincel A., Paredes E., Vidaud C., Malard V., Bresson C., Ortega R. (2021), NeuroToxicology, 82, 35-44. [link]
2020
How much manganese is safe for infants ? A review of the scientific basis of intake guidelines and regulations relevant to the manganese content of infant formulas
Mitchell E.J., Frisbie S.H., Roudeau S., Carmona A., Ortega R. (2020), Journal of Trace Elements in Medicine and Biology, 126710. [link]
Native separation and metallation analysis of SOD1 protein from the human central nervous system : A methodological workflow
Roudeau S., Trist B.G., Carmona A., Davies K.M., Halliday G.M., Rufin Y., Claverol S., Van Malderen S.J.M., Falkenberg G., Double K.L., Ortega R. (2020), ChemRxiv. [link]
Correlating STED and synchrotron XRF nano-imaging unveils cosegregation of metals and cytoskeleton proteins in dendrites
Domart F., Cloetens P., Roudeau S., Carmona A., Verdier E., Choquet D., Ortega R. (2020), eLife, 9:e62334. [link]
Estimating daily intakes of manganese due to breast milk, infant formulas, or young child nutritional beverages in the United States and France : Comparison to sufficiency and toxicity thresholds
Mitchell E.J., Frisbie S.H., Roudeau S., Carmona A., Ortega R. (2020), Journal of Trace Elements in Medicine and Biology, 62:126607. [link]
2019
Manganese levels in infant formula and young child nutritional beverages in the United States and France : comparison to breast milk and regulations
Frisbie S.H., Mitchell E.J., Roudeau S., Domart F., Carmona A., Ortega R. (2019), PLoS ONE, 14(11) : e0223636. [link]
Mapping chemical elements and iron oxidation states in the substantia nigra of 6-hydroxydopamine lesioned rats using correlative immunohistochemistry with proton and synchrotron micro-analysis
Carmona A., Roudeau S., Perrin L., Carcenac C., Vantelon D., Savasta M., Ortega R. (2019), Frontiers in Neuroscience, 13:1014. [link]
Manganese mapping using a fluorescent Mn2+ sensor and nanosynchrotron X-ray fluorescence reveals the role of the Golgi apparatus as a manganese storage site
Das S., Carmona A., Khatua K., Porcaro F., Somogyi A., Ortega R., Datta A. (2019), Inorganic Chemistry, 58, 13724-13732. [link]
Isotopic variations of copper at the protein fraction level in neuronal human cells exposed in vitro to uranium
Paredes Paredes E., Malard V., Vidaud C., Avazeri E., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2019), Analyst, 144, 5928-5933. [link]
Deciphering the uranium-target proteins in human dopaminergic SH-SY5Y cells
Vidaud C., Robert M., Paredes E., Ortega R., Avazeri E., Jing L., Guigonis J.M., Bresson C., Malard V. (2019), Archives of Toxicology, 93, 2141-2154. [link]
Emerging chemical tools and techniques for tracking biological manganese
Das S., Khatua K., Rakshit A., Carmona A., Sarkar A., Bakthavatsalam S., Ortega R., Datta A. (2019), Dalton Transactions, 48, 7047-7061. [link]
SLC30A10 mutation involved in parkinsonism results in manganese accumulation within nano-vesicles of the Golgi apparatus
Carmona A., Zogzas C.E., Roudeau S., Porcaro F., Garrevoet J., Spiers K., Salome M., Cloetens P., Mukhopadhyay S., Ortega R. (2019), ACS Chemical Neuroscience, 10, 1, 599-609. [pubmed] [link]
2018
Uranium exposure of human dopaminergic cells results in low cytotoxicity, accumulation within sub-cytoplasmic regions, and down regulation of MAO-B
Carmona A., Malard V., Avazeri E., Roudeau S., Porcaro F., Paredes E., Vidaud C., Bresson C., Ortega R. (2018), NeuroToxicology,
68, 177-188. [pubmed] [link]
Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
Paredes E., Avazeri E., Malard V., Vidaud C., Reiller P.E., Ortega R., Nonell A., Isnard H., Bresson C. (2018), Scientific Reports,
8, 17163. [link]
Advances in element speciation analysis of biomedical samples using synchrotron-based techniques
Porcaro F., Roudeau S., Carmona A., Ortega R. (2018), Trends in Analytical Chemistry,
104, 22-41. [link]
A new procedure for high precision isotope ratio determinations of U, Cu and Zn at nanogram levels in cultured human cells : what are the limiting factors ?
Paredes E., Avazeri E., Malard V., Vidaud C., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2018), Talanta,
178, 894-904. [link]
2017
Amyotrophic lateral sclerosis-like superoxide dismutase 1 proteinopathy is associated with neuronal loss in Parkinson’s disease brain
Trist B.G., Davies K.M., Cottam V., Genoud S., Ortega R., Roudeau S., Carmona A., De Silva K., Wasinger V., Lewis S.J.G., Sachdev P., Smith B., Troakes C., Vance C., Shaw C., Al-Sarraj S., Ball H.J., Halliday G.M., Hare D.J., Double K.L. (2017), Acta Neuropathologica,
134, 113-127. [pubmed]
[link]
Zinc and copper effects on stability of tubulin and actin networks in dendrites and spines of hippocampal neurons
Perrin L., Roudeau S., Carmona A., Domart F., Petersen J.D., Bohic S., Yang Y., Cloetens P., Ortega R. (2017), ACS Chemical Neuroscience,
8, 1490-1499. [pubmed]
[link]
Heterogeneous intratumoral distribution of gadolinium nanoparticles within U87 human glioblastoma xenografts unveiled by micro-PIXE imaging
Carmona A., Roudeau S., L’Homel B., Pouzoulet F., Bonnet-Boissinot S., Prezado Y., Ortega R. (2017), Analytical Biochemistry,
523, 50-57. [pubmed]
[link]
An Akouemma hemisphaeria organic macrofossils colony hosting biodiversity assemblage on the seafloor of Okondja Basin (Gabon) dated at 2.2 Ga
Edou-Minko A., Moussavou M., Sato T., Tchikoundzi C., Sawaki Y., Ndong Ondo S., Ortega R., Maire R., Kaestner A., Mbina Mounguengui M., Roudeau S., Fleury G., Carmona A., de Parseval Ph., Mvoubou M., Moussavou B.M., Agondjo M.O., Sasaki O., Maruyama S. (2017), Journal of Geology and Geophysics,
6, 281.
[link]
Growth, duplication and lateral mutual compressive deformation of Akouemma hemisphaeria on the seafloor of Okondja Basin at 2.2 Ga (Gabon)
Edou-Minko A., Moussavou M., Sato T., Sawaki Y., Ndong Ondo S., Maire R., Fleury G., Mbina Mounguengui M., Kaestner A., Ortega R., Roudeau S., Carmona A., Mvoubou M., Moussavou B.M., Sasaki O., Maruyama S. (2017), International Journal of Geosciences,
8, 1172-1191.
[link]
2016
Evidence of isotopic fractionation of natural uranium in cultured human cells
Paredes E., Avazeri E., Malard V., Vidaud C., Reiller P., Ortega R., Nonell A., Isnard H., Chartier F., Bresson C. (2016), Proc. Natl. Acad. Sci. USA.,
113, 14007-14012. [pubmed]
[link]
α-Synuclein
over-expression induces increased iron accumulation and redistribution
in iron-exposed neurons
Ortega R., Carmona A., Roudeau S., Perrin L., Dučić T., Carboni E.,
Bohic S., Cloetens P., Lingor P. (2016), Molecular Neurobiology,
53, 1925-1934. [pubmed]
[link]
Overview of chemical
imaging methods to address biological questions
Menezes
Lyra da Cunha M., Trepout S., Messaoudi C., Wu T-D, Ortega R.,
Guerquin-Kern J-L., Marco S. (2016), Micron, 84, 23-36. [pubmed]
[link]
Sod1 aggregation : A pathological link between Parkinson’s disease and amyotrophic lateral sclerosis ?
Trist B.G., Davies K.M., Genoud S., Smoothy V., Halliday G.M., Roudeau S., Carmona A., Perrin-Verdugier L., Ortega R., Double K.L. (2016), Movement Disorders,
31, S241-S242. [link]
2015
Evaluation of sample
preparation methods for single cell quantitative
elemental imaging using proton or synchrotron radiation focused beams
Perrin
L., Carmona A., Roudeau S., Ortega R.
(2015), Journal of Analytical Atomic
Spectrometry, 30, 2525-2532. [link]
Reduced net charge and heterogeneity
of pI isoforms in familial amyotrophic lateral sclerosis mutants of
copper/zinc superoxide dismutase
Roudeau S, Chevreux S, Carmona A, Ortega R. (2015), Electrophoresis,
36, 2482-2488. [pubmed]
[link]
Multicellular Consortia
Preserved in Biogenic Ductile-Plastic Nodules of Okondja Basin (Gabon)
by 2.1 Ga
Moussavou M., Edou-Minko A., Mbina Mounguengui M., Ortega R., Fleury
G., Roudeau S., Carmona A., Genty D., Blamart D., Tchikoundzi C.,
Makaya Mvoubou., Musavu Moussavou B., Ndong Ondo., Ogandaga Agondjo M.,
Dewilde F., Delorme G., de Parseval Ph., Weil R., Maire R. (2015), Journal of Geology &
Geophysics, 4:195.
[link]
Analysis of platinum and
trace metals in treated glioma rat cells by X-ray fluorescence emission
Gil S., Carmona A., Martínez-Criado G., León A., Prezado Y., Sabés M.
(2015), Biological
Trace Element Research, 163, 177-183. [pubmed]
[link]
2014
Correlative organelle
fluorescence
microscopy and synchrotron X-ray chemical element imaging in single
cells
Roudeau
S., Carmona A., Perrin L., Ortega R.
(2014), Analytical and
Bioanalytical Chemistry, 406, 6979-6991. [pubmed] [link]
Low-solubility particles
and a Trojan-horse type mechanism of toxicity : the case of cobalt
oxide on human lung cells
Ortega R., Bresson C., Darolles C., Gautier C., Roudeau
S., Perrin L.,
Janin M., Floriani M., Aloin V., Carmona A., Malard V. (2014), Particle & Fibre
Toxicology, 11:14. [pubmed] [link]
Stratigraphic analysis of
the Sterkfontein StW 573 Australopithecus skeleton and implications for
its age
Bruxelles L., Clarke R.J., Maire R., Ortega R., Stratford
D. (2014), Journal of
Human Evolution,
70, 36-48. [link]
Environmental manganese
compounds accumulate as Mn(II) within the Golgi
apparatus of dopamine cells : relationship between speciation,
subcellular distribution, and cytotoxicity
Carmona A., Roudeau S., Perrin L., Veronesi G., Ortega R.
(2014), Metallomics,
6, 822-832. [pubmed] [link]
Copper pathology in
vulnerable brain regions in Parkinson’s disease
Davies K.M., Bohic S., Carmona A., Ortega R., Cottam V.,
Hare D.J.,
Finberg J.P.M., Reyes S., Halliday G.M., Mercer J.F.B, Double K.L.,
(2014), Neurobiology of Ageing,
35, 858-866. [pubmed] [link]
Improvement and recent
applications of the Tohoku microbeam system
Matsuyama S., Ishii K., Watanabe K., Terakawa A., Kikuchi
Y., Fujiwara
M., Sugai H., Karahashi M., Nozawa Y., Yamauchi S., Fujisawa M., Ishiya
M., Nagaya T., Ortega R., Carmona A., Roudeau S. (2014), Nuclear Instruments and Methods
in Physics Research B, 318, 32-36. [link]
2013
Analytical tools for
speciation in the field of toxicology
Bresson C., Chartier F., Ansoborlo E., Ortega R.
(2013) .
Radiochimica Acta,
101, 349-357. [link]
Cobalt chloride
speciation, mechanisms of cytotoxicity on human pulmonary cells, and
synergistic toxicity with zinc
Bresson C., Darolles C., Carmona A., Gautier C., Sage N.,
Roudeau S., Ortega R., Ansoborlo E., Malard V. (2013), Metallomics, 5,
133-143. [pubmed] [link]
2012
Study of radiation
effects on the cell structure and evaluation of a dose delivered by
X-ray and alpha-particles microscopy
Kosior E., Cloetens P., Devès G., Ortega R., Bohic S.
(2012), Applied Physics
Letters, 101, 263102 [link]
X-ray absorption
spectroscopy of biological samples. A tutorial
Ortega R., Carmona A., Llorens I., Solari P.L. (2012), Journal of Analytical Atomic
Spectrometry, 27, 2054-2065 [link]
Special issue
on toxicology of pesticides
Sanchez-Bayo F., Ortega R. (2012), Journal of Environmental
Analytical Toxicology, S4-e001 [link]
High spatial resolution
chemical imaging of inorganic and organometallic pesticides
Ortega R., Carmona A. (2012), Journal of Environmental
Analytical Toxicology, S4-005 [link]
Micro-PIXE for single
cell analysis
Ortega R. (2012), International
Journal of PIXE, 22, 51-56 [link]
Coupling of native IEF
and Extended X-ray Absorption Fine Structure to
characterize zinc binding sites from pI isoforms of SOD1 and A4V
pathogenic mutant
Chevreux S., Llorens I., Solari P.-L., Roudeau S., Devès
G., Carmona A., Testemale D., Hazemann J.L., Ortega R. (2012), Electrophoresis,
33, 1276-1281 [pubmed] [link]
Combined use of hard
X-ray phase contrast imaging and X-ray fluorescence microscopy for
subcellular metal quantification
Kosior E., Bohic S., Suhonen H., Ortega R., Devès G.,
Carmona A., Marchi F., Guillet J.F., Cloetens P. (2012), Journal of Structural Biology,
177, 239-247. [pubmed] [link]
Chemical element imaging
for speleothem geochemistry : Application to
an uranium-bearing corallite with aragonite diagenesis to opal (Eastern
Siberia, Russia)
Devès G., Perroux A.-S., Bacquart T., Plaisir C., Rose,J.,
Jaillet S., Ghaleb B., Ortega R., Maire R. (2012)
, Chemical Geology,
294-295, 190-202 [link]
2011
Morphosedimentary study
of a cave deposit at Bois du Clos cave
(Charente, France) : geometric constraints and hydrological dynamics
Dandurand G., Devès G., Maire M., Ortega R., Genty D,
Ghaleb B. (2011), Quaternaire,
22, 285-306. [link]
The plant cell nucleolus
as a hot spot for iron
Roschzttardtz H., Grillet L., Isaure M.P., Conéjéro G.,
Ortega R., Curie C., Mari S. (2011),
The Journal of
Biological Chemistry, 286, 27863-27866. [pubmed] [link]
First quantitative
imaging of organic fluorine within angiogenic
tissues by Particle Induced Gamma-Ray Emission (PIGE) analysis : first
PIGE organic fluorine imaging
Lavielle S., Gionnet K., Ortega R., Devès G., Kilarski V.,
Wehbe K., Bikfalvi A., Déléris G. (2011), Pharmaceutics, 3,
88-106. [pubmed] [link]
Direct speciation
analysis of inorganic elements in single cells using X-ray absorption
spectroscopy
Ortega R. (2011),
Journal of Analytical
Atomic Spectrometry, 26, 23-29. [link]
X-ray fluorescence
microchemical analysis and autoradiography applied
to cave deposits : speleothems, detrital rhythmites, ice and
prehistoric paintings
Dandurand G., Maire M., Ortega R., Devès G., Lans B.,
Morel L., Perroux
A.-S., Vanara N., Bruxelles L., Jaillet S., Billy I., Martinez P.,
Ghaleb B, Valla F. (2011),
Géomorphologie : relief,
processus, environnement, 4, 407-426. [link]
2010
Manganese accumulates
within Golgi apparatus in dopaminergic cells as revealed by synchrotron
X-Ray fluorescence nano-imaging
Carmona A., Devès G., Roudeau S., Cloetens P., Bohic S.,
Ortega R. (2010), ACS
Chemical Neurosciences, 1, 194-203. [pubmed] [link]
Direct speciation
analysis of arsenic in sub-cellular compartments using micro-X-ray
absorption spectroscopy
Bacquart T., Devès G., Ortega R. (2010), Environmental Research,
110, 413-416. [pubmed] [link]
2009
3D imaging of human cells
by using PIXE-µ-CT
Kawamura Y., Ishii K., Matsuyama S., Nakhostin M.,
Fujiwara M., Tsuboi
S., Yamanaka K., Watanabe M., Okura S., Hashimoto Y., Fujikawa M.,
Hatori Y., Hamada N., Fujiki K., Yamazaki H., Ortega R., Deves G.,
Carmona A. (2009), International
Journal of PIXE, 1-2, 29-37. [link]
EXAFS analysis of a human
Cu,Zn SOD isoform focused using non-denaturing gel electrophoresis
Chevreux S., Solari P.L., Roudeau S., Devès G., Alliot
I.,Testemale J.L., Hazemann J.L. Ortega R. (2009), Journal of Physics IV,
190, 012205. [link]
Multimodal analysis of
metals in copper-zinc superoxide dismutase isoforms separated on
electrophoresis gels
Chevreux S., Roudeau S., Fraysse A., Carmona A., Devès G.,
Solari P.-L., Mounicou S., Lobinski R., Ortega R. (2009), Biochimie 91,
1324-1327. [pubmed] [link]
Bio-metals imaging and
speciation in cells using proton and synchrotron radiation X-ray
micro-spectroscopy
Ortega R., Devès G., Carmona A. (2009), J. R. Soc. Interface
6, S649–S658. [pubmed] [link]
Fluorine micro-imaging
and quantification using nuclear reaction
analysis : a new tool for tissue distribution validation of positron
emission tomography tracers
Devès G., Roudeau S., Carmona A., Lavielle S., Gionnet K.,
Déléris G., Ortega R. (2009), Applied
Physics Letters 95, 023701. [link]
Cobalt distribution
in keratinocyte cells indicates nuclear and perinuclear accumulation
and interaction with magnesium and zinc homeostasis
Ortega R., Bresson C., Fraysse A., Sandre C., Devès G.,
Gombert C.,
Tabarant M., Bleuet P., Seznec H., Simionovici A., Moretto Ph., Moulin
C. (2009), Toxicology
Letters 188, 26-32. [pubmed] [link]
Synchrotron radiation for
direct analysis of metalloproteins on electrophoresis gels
Ortega R. (2009), Metallomics,
1, 137-141. [pubmed] [link]
Public health
strategies for Western Bangladesh that address the arsenic, manganese,
uranium and other toxic elements in their drinking water
Frisbie S.H., Mitchell E.J., Mastera L.J., Maynard D.M.,
Zaki Yusuf A.,
Yusuf Siddiq M., Ortega R., Dunn R.K., Westerman D.S., Bacquart T.,
Sarkar B. (2009), Environmental
Health Perspectives, 117, 410-416. [pubmed] [link]
2008
Direct speciation of
metals in copper-zinc superoxide dismutase
isoforms on electrophoresis gels using X-ray absorption near edge
structure
Chevreux S., Roudeau S., Fraysse A., Carmona A., Devès G.,
Solari P.L., Weng T.C., Ortega R. (2008), Journal of Analytical Atomic
Spectrometry, 23, 1117-1120. [link]
Nano-imaging of trace
metals by synchrotron X-ray fluorescence into dopaminergic single cells
and neurite-like processes
Carmona A., Cloetens P., Devès G., Bohic S., Ortega R.
(2008), Journal of
Analytical Atomic Spectrometry, 23, 1083-1088. [link]
Development of analytical
procedures for the determination of
hexavalent chromium in corrosion prevention coatings used in the
automotive industry
Séby F., Castetbon A., Ortega R., Guimon C., Niveau F.,
Barrois-Oudin N., Garraud H., Donard O.F. (2008), Analytical Bioanalytical
Chemistry, 391, 587-597. [pubmed] [link]
Quantitative
micro-analysis of metal ions in subcellular compartments
of cultured dopaminergic cells by combination of three ion beam
techniques
Carmona A., Devès G., Ortega R. (2008), Analytical Bioanalytical
Chemistry, 390, 1585-1594. [pubmed] [link]
Micro-chemical imaging of
iodine distribution in the brown alga
Laminaria digitata suggests a new mechanism for its accumulation
Verhaeghe E., Fraysse A., Guerquin-Kern J-L., Wu T.D.,
Devès G.,
Mioskowski C., Leblanc C., Ortega R., Ambroise Y., Potin P. (2008), Journal of Biological Inorganic
Chemistry, 13, 257-269. [pubmed] [link]
2007
Iron storage in
neurovesicles revealed by chemical nano-imaging
Ortega R., Cloetens P., Devès G., Carmona A., Bohic S.
(2007), PLoS ONE,
2(9), e925. [pubmed] [link]
Subcellular speciation
analysis of trace element oxidation states using
synchrotron radiation micro-X-ray absorption near edge structure
Bacquart T., Devès G., Carmona A., Tucoulou R., Bohic S.,
Ortega R. (2007),
Analytical Chemistry,
79, 7353-7359. [pubmed] [link]