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iRiBio team

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Hervé SEZNEC is a researcher at CNRS / IN2P3. Head of the iRiBio team, Hervé Seznec is dedicated to the development and implementation of physical methods using ion microbeams in order to adress fundamental and applied questions in the field of life sciences. He develops multi-modal and multiscale approaches in order to obtain qualitative, quantitative, dynamic and real-time molecular and cellular data at the individual and population level.

As a biologist (PhD in Biology and Molecular Genetics), he uses biological models like Caenorhabditis elegans to study precisely the molecular and cellular mechanisms induced by physical stresses (ionizing radiation, nanoparticles of metal oxides). Today, his research is based on a close combination of cellular and molecular biology methods and on the strong technical potential of these instruments in particle physics (ion microbeam) to characterize the biological responses induced by low doses of exposure to ionizing radiation (micro-irradiation controlled at the subcellular scale) and / or exposures to nanoparticles of metal oxides (quantitative and multi-modal micro-analysis).

His research falls within the following fields of application: (i) knowledge of the fundamental mechanisms involved in maintaining the integrity of genomes; (ii) molecular and cellular radiobiology; (iii) correlative and multi-modal microscopy; (iv) new anti-cancer therapeutic approaches (proton therapy, nanomedicine).
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Dr Hervé Seznec

Head of the iRiBio group
CNRS researcher - HDR

CENBG - iRiBio
Chemin du solarium
CS10120 - 33175 Gradignan cedex
Tel: +33 (0)557 120 864
Email: seznech at cenbg.in2p3.fr

Expertise

Cell and molecular biology (transfection, QRTPCR, ...), microbiology (Caenorhabditis elegans), real-time video microscopy, confocal microscopy, ion beam microanalysis, correlative microscopy

Keywords

: DNA repair, cellular and molecular radiobiology, nanomedicine, nanotoxicology, microscopy, Caenorhabditis elegans
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As part of the interdisciplinary research programs of the iRiBio group, Philippe Barberet is dedicated to the development of experimental tools (ion microbeams) and numerical tools (Monte-Carlo simulations) derived from nuclear instrumentation and applied to life sciences. As a physicist, P. Barberet joined the team in 2006 after a doctorate at CENBG (2000-2003) and two years of post-doctoral studies at GSI Darmstadt in Germany (2004-2006). P. Barberet is particularly involved in the development of the focused beamlines of the AIFIRA platform. These experimental methods of targeted micro-irradiation and elemental micro-analysis are used to characterize radiation-induced biological responses at the cellular level and the impact of exposure to nanoparticles of metal oxides on the living.

Expertise

Ion microbeams, accelerators, nuclear instrumentation, microscopy, elemental microanalysis, Monte Carlo simulations (Geant4), diamond detectors
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Dr Philippe Barberet

Associate professor, Bordeaux university

CENBG - iRiBio
Chemin du solarium
CS10120 - 33175 Gradignan cedex
Tel: +33 (0)557 120 903
Email: barberet at cenbg.in2p3.fr

Teaching

Head of the Master of Engineering in Physics: Radiation and Instrumentation" Board Member of the Physics Training Unit Courses, tutorials and practical work in physics and physics-chemistry at Bordeaux University.
Courses and experimental work in master of physics and master "Materials of Cultural Heritage and Archeometry" (University Bordeaux-Montaigne): nuclear physics, ion beam analysis, radiation-matter interaction, medical nuclear physics, data acquisition, projects in instrumentation.
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Expertise

  • Ion beam analysis PIXE, STIM, RBS
  • Chemical imaging by µ-XRF and synchrotron x-ray fluorescence
  • Chemical element speciation using XANES, EXAFS
  • Epi-fluorescence microscopy and confocal microscopy
  • Statistics and Data analysis

Curriculum

  • PhD in analytical chemistry and environment - University of Bordeaux (2010)
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Dr Guillaume Devès

Research engineer

CENBG - iRiBio
Chemin du solarium
CS10120 - 33175 Gradignan cedex
Tel: +33 (0)557 120 903
Email: deves at cenbg.in2p3.fr
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Expertise


  • Extension of Geant4 as part of the Geant4-DNA project for the modeling of direct and indirect early damage caused by ionizing radiation.
  • Modeling of realistic cellular geometries based on 3D confocal imaging
  • Modeling of specific radiative environments (eg aboard the International Space Station, on the surface of the planet Mars) for the development of biochips for the search for traces of life in the solar system. Participation in associated irradiation campaigns.
  • Beam optics with high focusing power
  • Leader of the development of low energy electromagnetic physics at Geant4.
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    Dr Sébastien Incerti

    CNRS research director
    Spokesman of the Geant4-DNA collaboration
    Director of the International French Korean laboratory FKPPL
    Member of CoNRS, section 01
    Associate editor ofEuropean Journal of Medical Physics - Physica Medica / Elsevier

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 889
    Email: incerti at cenbg.in2p3.fr

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    Expertise

    • Development of STIM and PIXE tomography techniques
    • Procedures for viewing and processing 3D data
    • 3D quantitative analysis

    Dr Claire Michelet

    Associate Professor, Bordeaux University Head of the Master 2 Pro Nuclear Instrumentation

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 864
    Email: michelet at cenbg.in2p3.fr
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    With Master degree in Medical Physics from Paul Sabatier University (Toulouse III), Eva TORFEH joined the iRIBIO group in November 2016 for her PhD in Physics at the University of Bordeaux (Graduate School of Physics and Physics engineering, PhD supervisor Philippe BARBERET). As part of the radiobiology studies carried out within the iRiBio group, Eva Torfeh’s thesis project aims to define and precisely characterize the dosimetry at the cellular level and the different physical parameters for experimental irradiations on human cells (in vitro) and on a living organism "C.elegans" (in vivo). Eva Torfeh is developing micro-dosimetric models in order to define different physical observables: local dose, energy deposits, secondary particle spectra, trace structure, etc ... The interest is to to be able to correlate these numerical simulations with the biological responses observed following the irradiations. These simulations are performed using the Monte Carlo Geant4 tool and its extension for micro-dosimetry Geant4-DNA).

    Expertise

    Monte-Carlo Simulation (Geant4 et Geant4-DNA), confocal microscopy et image processing (ImageJ), culture and maintenance of C.elegans, data processing (Python)

    Eva Torfeh

    PhD student

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 864
    Email: torfeh at cenbg.in2p3.fr
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    Wook Geun Shin is a doctoral student in co-supervision between the University of Bordeaux and the University of Yonsei in South Korea (2017-2020). Its activity focuses on the development of new physical models for Geant4-DNA and the extension of Geant4-DNA for the modeling of early and late biological damage. He is a member of the France-South Korea FKPPL Associated International Laboratory.
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    Wook-Geun Shin

    PhD student

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 889
    Email: shin at cenbg.in2p3.fr
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    With Master’s Degree in Medical Biotechnology from the University of Turin, Giovanna Muggiolu joined the iRIBIO group in September 2013 for her PhD thesis in Cell Biology and Physiopathology at the University of Bordeaux (Doctoral School of Health Biology, PhD Direction H. Seznec). Giovanna has been recruited as part of the SPRITE European Marie Curie Program (FP7). SPRITE is a European network whose objective is to promote the exchange between ion beam platforms at European level and to promote the transfer of these technologies to the industrial and / or biomedical (SPRITE) world. As part of the SPRITE program, interdisciplinary collaborations are strongly encouraged, including the implementation of complementary experimental approaches: (i) cellular and molecular biology, (ii) radiobiology, (iii) medical physics and radiotherapy, (iv) oncology molecular, (v) nanotechnologies ... Giovanna’s thesis project is carried out in close collaboration between the iRIBIO team, the radiotherapy department of the Bergonié Institute (Prof. G. Kantor) and the "Sarcome" team of the Bergonié Institute (F. Chibon, INSERM / VINCO). For Giovanna, this involves developing and validating in vitro evaluation protocols for the radiosensitivity of a collection of cancer cell lines established from patients with sarcomas. In addition to studying the relationship between genetics and response to ionizing radiation, Giovanna will also compare the radiation-induced biological effects produced by different types of radiation (electrons versus protons). To do this, Giovanna shares her activity time between the Bergonié Institute and the CENBG (AIFIRA platform).

    Expertise

    Radiobiology, Cellular and molecular biology, DNA repair, cancer / sarcomas, microscopy and image processing (ImageJ), charged particle beam micro-irradiation (protons).
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    Giovanna Muggiolu

    PhD student (2013-2016)

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 864
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    Dr. Dousatsu Sakata is now an associate researcher at Wollongong University in Australia. After a two-year stay in the iRiBio team during which he developed the first models of Geant4-DNA describing the physical interactions of electrons in nanoscale gold targets, he is now investing in modeling and experimental study of the use of gold nanoparticles in a radiotherapy context, in collaboration with the iRiBio team.
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    Dr Dousatsu Sakata

    Researcher

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
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    Post-doctoral researcher, Marina Simon holds a PhD in Cellular Biology and Physiopathology from the University of Bordeaux physiopathology (doctoral school Biology Health, 2006-2009). His thesis topic concerned the study of the relationship between ionic homeostasis and cutaneous cell functions (under ex vivo and in vitro conditions) in response to different physiological stress (ex-vivo explant culture conditions, physical stress and / or chemical, exposures to nanoparticles of metal oxides) 1-4. This work was done in collaboration with the Pierre Fabre Research Institute (Toulouse). As part of these doctoral studies, Marina has exploited all the techniques of chemical micro-analysis offered by the ion beams and applied to Life Sciences (nuclear microprobe). In order to continue this work, Marina had the opportunity to join Pr. Hovnanian’s team at the Necker Institute IMAGINE (U1163 INSERM / Paris) to study the consequences of a deregulation of calcium homeostasis within of an autosomal dominant cutaneous genetic disease, Darier’s disease. During this first postdoctoral fellowship, Marina focused her work on the study of endoplasmic reticulum stress and sought to identify, by transcriptomic analysis (microarrays), new signaling pathways involved in the pathophysiology of Darier’s disease5 6. Marina recently joined the radiotherapy department of the Institut Bergonié and the iRibio team, to precisely study the genetic, molecular and cellular consequences induced in response to new radiotherapy protocols combining irradiation (protons, electrons) and the use of radiotherapy. nanoparticles of metal oxides. This in vitro study is carried out on sarcoma lines (established by F. Chibon, INSERM / VINCO team) and in parallel with clinical trials carried out at the Institut Bergonié, in close collaboration with Nanobiotix.
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    Dr Marina Simon

    Researcher

    CENBG - iRiBio
    Chemin du solarium
    CS10120 - 33175 Gradignan cedex
    Tel: +33 (0)557 120 903
    Email: simon at cenbg.in2p3.fr

    Expertise

    cell and molecular biology (primary cell culture, lineages, RT-qPCR ...), high-throughput genomic analysis / microarrays analysis. Biochemistry (Western blot, immunofluorescence, sucrose gradient ...), epifluorescence and confocal microscopy, ion beam microanalysis.

    Keywords

    nanomedicine, nanotoxicology, microscopies, ionizing radiation, ion microbeams (PIXE, STIM), nanoparticles, protons, ionic homeostasis, calcium metabolism, endoplasmic reticulum stress.

    References
    1. Incerti, S. et al. Monte Carlo dosimetry for targeted irradiation of individual cells using a microbeam facility. Radiat. Prot. Dosimetry 133, 2–11 (2009).
    2. Simon, M. et al. The skin barrier function: a micro-PIXE study. X-Ray Spectrom. 38, 132–137 (2009).
    3. Simon, M., Barberet, P., Delville, M.-H., Moretto, P. & Seznec, H. Titanium dioxide nanoparticles induced intracellular calcium homeostasis modification in primary human keratinocytes. Towards an in vitro explanation of titanium dioxide nanoparticles toxicity. Nanotoxicology 5, 125–139 (2011).
    4. Barberet, P. et al. First results obtained using the CENBG nanobeam line: Performances and applications. Nucl. Instruments Methods Phys. Res. Sect. B Beam Interact. with Mater. Atoms 269, 2163–2167 (2011).
    5. Savignac, M., Edir, A., Simon, M. & Hovnanian, A. Darier disease: A disease model of impaired calcium homeostasis in the skin. Biochimica et Biophysica Acta - Molecular Cell Research 1813, 1111–1117 (2011).
    6. Savignac, M., Simon, M., Edir, A., Guibbal, L. & Hovnanian, A. SERCA2 Dysfunction in Darier Disease Causes Endoplasmic Reticulum Stress and Impaired Cell-to-Cell Adhesion Strength: Rescue by Miglustat. J. Invest. Dermatol. 1–10 (2014). at
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    Dr Philippe Moretto

    Directeur du CENBG
    Professeur

    Email: moretto at cenbg.in2p3.fr
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