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Accueil du site > ANGLAIS > AIFIRA Home > About AIFIRA Facility > Microbeam line


Microbeam line

The microbeam line replaces the former microprobe which was in function since the beginning of the 90’s. Same as the former version, the microbeam line allows the perceptive analysis of a lot of materials, from biological samples of few micron size to geological samples of millimetre-length dimensions.

 

Focusing device

 

The adjustments of the system object chamber / electromagnetic lenses (Q-poles) allows the ion beam focusing. The microbeam is obtained in "triplet" configuration that corresponds to a set of three Q-pôles situated just before the analysis chamber. In this configuration, an object of 100 µm placed in the object chamber gives a spot of about 5 µm in the analysis chamber after adjustments.

 

The working beam size for PIXE analysis is below 1 µm for a integrated current of about 300 pA on the samples. For STIM analysis, the beam size is around 350 nm for a rate of 1500 counts/s recorded on the STIM detector.

 

The "quintuplet" set is the addition of 2 more Q-poles ("doublet") to the "triplet" system, situated in the middle of beam line. This set is used to obtain a sub-micronic beam size during STIM analyses. The size of the beam for an object of 5 µm is then of 200 nm for a rate of 500 counts/s on the STIM detector.

 

Microbeam chamber

 

The scanning device allows the mapping by RBS, PIXE, NRA, ERDA and STIM of specific zone of the sample. Its width goes from a few tens of microns to 1 millimeter accordingly to the energy of the beam.

 

The sample holder is motorized to easily and precisely analyse a large number of samples. A specific sample holder provided with a goniometer also motorized, can be settled in the chamber in order to perform PIXE tomography and ERDA. Several holders are available according to the shape and the nature of samples (massif, cells, thin films...)

 

Example of holders available for the analysis
DSCF1489_a DSCF1489_b

 

 

 

 

 

 

The observation of the samples are done through three objectives in transmission mode (X4 and X20 magnification) and in reflection mode (from x10 to x20 magnification).

 

Three detectors are permanently present:

    ♦ a Silicon junction (25 or 50 mm 2) for RBS placed in 135 ° below the beam axis

    ♦ two PIXE detectors (Be windows of 8 mm, Li-doped Si crystal)) placed in 135° on both sides of the beam axis

 

Furthermore, a Silicon junction for STIM can be placed in the axis of the beam, in transmission mode and an annular Silicon detector (surface 150 mm 2 can also be mounted mostly in order to perform nuclear reaction or RBS analysis.

 

DSCF1463_b {PNG}

The microbeam chamber inside

 

Applications

 

The most common analyses are made on biological samples because of the very good resolution and brightness available on the microbeam line.

 

The presence of a remote control also offers the possibility to make analysis with a deuton beam essentially used for the study of materials for energy.

 

Communications

With the new microbeam line

    2012

S. Chevreux et al. Electrophoresis 33, 1276-1281

E. Kosior et al. J Structural Bio 177, 239-247

    2011

A. Carmona et al. Golgi apparatus functions in manganese homeostasis and detoxification. In: Golgi apparatus: structure, functions and mechanisms. Chap 5, C.J. Hawkins (Ed), Nova Science Publishers Inc, New York

J. Isaac et al. Eur. Cells & Mat. 21, 130-143

 

    2010

R. Baillot et al. Microelectronics Reliab 50, 1568–1573

E. Jallot et al. App. Mater & Interfaces 2, 1737-42

 

    2009

P. Barberet et al. Nuc Instr Meth Phys Res B 267, 2003-2007

 

    2008

F. Andersson et al. Nuc Instr Meth Phys Res B 266, 1653-1658

 

    2007

S. Incerti et al. Nuc Instr Meth Phys Res B 260, 20-27

 

    2006

S. Incerti et al. Nuc Instr Meth Phys Res B 249, 738-742

 

With the "old" microbeam line

 

    2011

S. Lavielle et al. Pharmaceutics 3, 88-106

M. Simon et al. Nanotoxicology, Mars 22

H. Roschzttardtz et al. J Biological Chem 286, 27863 - 27866

 

    2010

L. Beck et al. Nuc Instr Meth Phys Res B 268, 2086-2091

A. Carmona et al. ACS Chem Neurosciences 1, 194-203

 

    2009

S. Chevreux et al. Biochimie 91, 1324-1327

G. Devès et al. Appl Phys Lett 95, 023701

C. Habchi et al. Nuc Instr Meth Phys Res B 267, 2107-2112

S. Incerti et al. Radiat Prot Dosimetry 133, 2-11

J. Lao et al. J Mater Chem 19, 2940-2949

R. Ortega et al. Toxicology Lett 188, 26-32

R. Ortega et al. J. R. Soc. Interface 6, S649-S658

M. Simon et al. X-Ray Spectrom 38, 132-137

J. Soulié et al. Phys Chem Chem Phys 11, 10473-10483

 

    2008

C. Bressy et al. Comptes Rendus Palevol 7, 237-248

A. Carmona et al. Anal bioanal Chem 390, 1585-1594

E. Gontier et al. Nanotoxicology 2, 218-231

C. Habchi et al. Revue des Questions Scientifiques 179, 217-240

J. Lao et al. Chem Mater 20, 4969-4973

J. Lao et al. J Phys Chem C 112, 9418-9428

J. Lao et al. Nuc Instr Meth Phys Res B 266, 2412-2417

J. Lao et al. Surf Interf anal 40, 162-166

C. Luglié et al. Comptes Rendus Palevol 7, 249-258

E. Verhaeghe et al. J Biol Inorg Chem 13, 257-269

 

    2007

J. Lao et al. Nuc Instr Meth Phys Res B 261, 488-493

C. Luglié et al. J Archaeol Sc 34, 428-439

R. Ortega et al. PLos One 2, e925

A. Verissimo et al. Microscopy Res Technique 70, 302-309

 

    2006

C. Bresson et al. Biochimie 88, 1619-1629

G. Guibert et al. Nuc Instr Meth Phys Res B 251, 246-256

C. Habchi et al. Nuc Instr Meth Phys Res B 249, 653-659

S. Incerti et al. Radiat Prot Dosimetry 122, 327-329

M.P. Issaure et al. Biochimie 88, 1583-1590

E. Jallot et al. Instr Sc Technol 34, 405-416

J. Lao et al. Nuc Instr Meth Phys Res B 245, 511-518

M.D. Ynsa et al. Nuc Instr Meth Phys Res B 249, 710-714